Figure 8
From: Cytosolic Ca2+ shifts as early markers of cytotoxicity
PAR formation and its effect on cell survival after lethal doses of As 2 O 3 , gossypol, H 2 O 2 and staurosporine in HeLa and MCF-7 cells. (A) PAR detection by immunofluorescence in HeLa cells treated with 50 μM As2O3, 100 μM gossypol, 2 mM H2O2 or 1 μM staurosporine as described in Methods. Nuclear DAPI staining is shown as control. (B) PAR detection by immunofluorescence in MCF-7 cells treated with 50 μM As2O3, 75 μM gossypol, 10 mM H2O2 or 0.4 μM staurosporine as described with nuclear DAPI staining as control. (C) Cell viability was assessed with Alamar Blue in HeLa cells in presence or absence of PJ-34 (5 μM). Cells were challenged with EC75 values of As2O3, gossypol, H2O2 and staurosporine as indicated. Differences of cell survival are shown (mean±SD; *p<0.05; n=8; t test). (D) Cell viability was assessed with Alamar Blue in MCF-7 cells in presence or absence of PJ-34 (5 μM). Cells were challenged with EC75 values of As2O3, gossypol, H2O2 and staurosporine as indicated. Differences of cell survival are shown (mean±SD; *p<0.05; n=8; t test).