Your privacy, your choice

We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media.

By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with varying standards of data protection.

See our privacy policy for more information on the use of your personal data.

for further information and to change your choices.
Skip to main content
Figure 2 | Cell Communication and Signaling

Figure 2

From: A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells

Figure 2

Analysis of cell cycle. Panel A: Photographs of hESC at different densities. The table below is an analysis of cell cycle profile using EdU for 30 minutes at 10 uM. Note the differences in G1 and G2/M with different cell density. Using cells at 50-60% confluence is optimal. Lower Panel: Histogram of hESC treated with BRDU demonstrating a 50-60% of cells in S phase. Panel B: Flow cytometry histograms of cell cycle profile of hESC, human IPS cells, mouse IPS cells and Jurkat cells treated with EdU and DAPI. Note the red box highlights the mean intensity of EdU signal that can also be used as an indicator of the proliferative state of the cells.

Back to article page

Cell Communication and Signaling

ISSN: 1478-811X