Figure 5
From: CD39 is a negative regulator of P2X7-mediated inflammatory cell death in mast cells
Stimulation with ATPγS aggravates cell death and IL-1β release. (A) wt and P2rx7−/− BMMCs were primed with 1 μg/ml LPS for 3.5 h and then left untreated or stimulated with the indicated concentrations [mM] of ATPγS for 1 h. TCL was probed for IL-1β by ELISA (n = 4). (B) Treatment as in (A); SN of wt and P2rx7−/− BMMCs were probed for IL-6 by ELISA (n = 4). (C) Treatment as in (A); SN of wt and P2rx7−/− BMMCs were probed for IL-1β by ELISA (n = 4). (D) Treatment as in (A); wt and P2rx7−/− BMMCs were stained with Pi and analyzed by FACS (n = 3). (E) Treatment as in (A); cells were stained with FITC-conjugated Annexin V and Pi and analyzed by FACS. The morphology is displayed in the forward and side scatter (FSC/SSC); representative result (n = 3). Shown are means and SD of replicates of one representative experiment each. Statistical analysis of n independent experiments by LMM; FDR-corrected p-values: * < 0.05, ** < 0.005, and *** < 0.0005.