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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Amino acid response by Halofuginone in Cancer cells triggers autophagy through proteasome degradation of mTOR

Fig. 2

Halofuginone induces autophagy. a Cells were exposed to halofuginone (HF) as in Fig. 1 and LC3B protein levels were assessed by immunoblotting. As loading control, filters were stripped and probed with ant β-Tubulin as loading control. Representative immunoblots are shown along with LC3B-II/I band intensity ratios as index of autophagosome formation. Histograms show mean (±SD) LC3B-II/I band intensity ratios of three different experiments. Statistically significant differences between LC3B-II/I ratios after to before HF are shown (*, p ≤ 0.05). b WRO cells were exposed to halofuginone (HF) for 8 h in the presence or in the absence of 30 μM chloroquine (CQ) and LC3B protein levels were assessed by immunoblotting. As loading control, filters were stripped and probed with anti β-Tubulin as loading control. Representative immunoblots are shown along with LC3B-II/I band intensity ratios as index of autophagosome formation. Relative amount of newly formed autophagosomes is expressed as LC3B-II/I ratios difference in the presence or absence of CQ (APH biogenesis). Histograms show mean (±SD) LC3B-II/I band intensity ratios of 3 different experiments. Statistically significant differences between LC3B-II/I ratios after to before HF are shown (*, p ≤ 0.05)

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Cell Communication and Signaling

ISSN: 1478-811X