Fig. 3

MAP4 hyperphosphorylation mutation leads to proteinuria and nephropathy reminiscent of diabetic kidney disease in MAP4 KI mice. A, B Representative WB (A) and quantitative analysis (B) depicting p-M (S737 and S760) in WT and MAP4 KI mice at 10–14 weeks. n = 6. C–F UACR, serum levels of CR, UN and Cys-c were determined in two mouse models at different ages. n = 8. G, H HE and Masson’s trichrome staining as well as glomerular volume quantitative analysis of deparaffinized kidney tissue section was examined at different ages. Bar, 20 µm. n = 6. I, J Quantitative analysis of foot processes fusion (I) and TEM observation (J). Bar, 2 µm. n = 6. K, L Podocyte apoptosis of frozen kidney tissue section was detected by WT-1 and TUNEL co-staining. Bar, 10 µm. n = 6. M, N Cell proliferation of frozen kidney tissue section was determined by EdU staining. Bar, 25 µm. n = 6. The graph showed mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. P values were derived from one-way ANOVA with Bonferroni’s post-test