Fig. 6

VSIG2 advanced PDAC progression through LAMTOR2-mediated mTOR activation. A CCK-8 assays were performed to detect the proliferation of PDAC cells regarding to down-regulation of VSIG2 and simultaneous elevation of LAMTOR2 or supplement of MHY1485, **P <0.01. B, C The properties of proliferation about PDAC cells according to different groups were demonstrated using colony formation assays, **P <0.01. D, E Transwell assays were used to certify the ability of migration and invasion about PANC-1 cells regarding to down-regulation of VSIG2 and simultaneous elevation of LAMTOR2 or supplement of MHY1485. F, G The related indicators of EMT were clarified by western blots after VSIG2 knockdown, LAMTOR2 overexpression and addition of mTOR activator MHY1485 in PANC-1 and AsPC-1 cells. GAPDH was utilized as a loading control. The bar charts showed statistical differences connected with migration and invasion PDAC cells, **P<0.01. H, I, J Subcutaneous xenograft tumor model was constructed to ulteriorly prove the conclusion, and tumor diameter was recorded once every three days, while tumor weight was investigated after dissection, **P<0.01