Fig. 4

SLIT3 deficiency is associated with increased osteoclastic potential under LPS stimulation. A, B WT and Slit3^−/− mice at 8 weeks of age were injected intraperitoneally with PBS or LPS. Both hind limbs were examined by micro-CT imaging. Representative images of the trabecular bone of femurs from each group are presented (n = 4 ~ 6 images taken in total, one image from each of 4 ~ 6 mice). Histograms representing the three-dimensional structural parameters of the femurs in a micro-CT reconstruction of the metaphyses of distal femurs (A). (B) Three-dimensional morphometric analysis of bone parameters including bone volume per tissue volume (BV/TV), trabecular number (Tb. N), trabecular thickness (Tb. Th), trabecular separation (Tb. Sp), structure model index (SMI), and total porosity (%) were calculated from femur sections using the micro-CT analysis program. The results are presented as the means ± s.d. of 4 to 6 male mice/group. C Hematoxylin and eosin (H/E) and TRAP (purple) staining of hind limb sections from WT and Slit3^−/− mice. Scale bar, 100 μm. Data are shown as mean with s.d., *P < 0.05, **P < 0.005, ***P < 0.0001. All representative data from three independent experiments are shown