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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Reciprocal regulation of TWIST1 and OGT determines the decitabine efficacy in MDS/AML

Fig. 5

TWIST1 activates OGT transcription. A, B Expression of OGT at protein (A) and mRNA (B) levels in KG1a, KG1a-TWIST1, SKM1 and SKM1-shTWIST1 cells. C ChIP analysis for TWIST1 binding to E-box motifs of OGT promoter (0–2000 bp). GAPDH was used as negative control. D Validation of TWIST1 binding to OGT promoter by EMSA. E DNA pull down assay. Biotinylated OGT promoter region was incubated with cell nucleus lysate, enriched by magnetic beads conjugated with streptavidin and analyzed by western blotting. F Putative TWIST1 binding sites on wild-type (WT) and mutant sequences of 4 E-box (OGT-M1, OGT-M2, OGT-M3 and OGT-M4) of OGT promoter by dual luciferase reporter assay. *p < 0.05, ***p < 0.001, ns: not significant

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Cell Communication and Signaling

ISSN: 1478-811X