Fig. 1

IRP2 regulated tumor cell growth in CRC cells. (A) Cell proliferation assay was performed on cells transfected with siRNA. Cells were monitored every 24 h, and six random fields were counted using the Harmony software. Data are represented as the mean ± SEM (n = 4). **p < 0.01, ***p < 0.001. (B) Cell proliferation assay was performed on IRP2 knockout cell lines. Cells were monitored every 24 h, and six random fields were counted using the Harmony software. IRP2 knockout was confirmed using immunoblotting. Data are represented as the mean ± SEM (n = 4). **p < 0.01, ***p < 0.001. (C) Representative tumor image of SW480 xenograft injecting IRP2 knockout cell (n = 4). (D) Tumor volumes were monitored once every two days for 44 days. Data are represented as the mean ± SEM (n = 4). **p < 0.01. (E) The tumors were isolated and weighed. Data are represented as the mean ± SEM (n = 4). *p < 0.05