Fig. 4

ONC212 induces Bcl-2/Bcl-xL inhibitable apoptosis in HeLa and A549 cancer cells. a. Western blot images of Bcl-2 family proteins (Bcl-2, Bcl-xL, Bax, and Bak) and TRAP1 expression in control and ONC212 treated cells. Antiapoptotic Bcl-2 and Bcl-xL proteins were enhanced whereas proapoptotic Bax and Bak were slightly reduced by ONC212 treatment in cancer cells. b. The nuclear condensation was assessed under a fluorescence microscope by Hoechst staining after ONC212 treatment in wild-type and Bcl-2/Bcl-xL GFP overexpressing cancer cells (48 h). The Percentage of condensed nuclei in random fields represented graphically (n = 3, mean ± SEM), ** and *denotes p < 0.01 and p < 0.05 respectively in both control vs ONC212 treated groups. c. Flow cytometry histogram demonstrating comparative cell death after ONC212 treatment in wild type vs Bcl-2 GFP and Bcl-xL-GFP overexpressing HeLa and A549 cancer cells using zombie red staining