Fig. 1

Effect of different concentrations of AKG on oocyte maturation. (A) Representative images showed the cow cumulus oocyte complex after 22 h of culture with different concentrations of AKG treatments, Scale bar = 200 µM, n = 31 (Control), 30 µM AKG n = 30 (AKG30), 150 µM AKG n = 31 (AKG150), 750 µM AKG n = 31 (AKG750) examined over three independent experiments. (B) Relative cumulus expansion area. (C) Cumulus expansion index. (D) The polar body extrusion (PBE) rate of oocytes after 22 h of maturation. The number inside each bar indicates the number of PBE oocytes/total oocytes. Data were analyzed using the Chi-squared test with SPSS. (E) Representative images of cytoskeleton staining of oocytes labeled with F-actin and the CGs dynamic staining with Lectin from Arachis, Scale bar = 70 µM, n = 23 (Control), n = 26 (AKG30), n = 23 (AKG750) examined over three independent experiments. (F) Degree of cytoskeleton assembly of oocytes, as measured by the relative fluorescence intensity of F-actin in the outer layer of oocytes. (G) Degree of cortical granules (CGs) dynamic in oocytes, determined by the relative fluorescence intensity in the outer layer of oocytes