Fig. 2

Effects of AKG on the levels of ROS and apoptosis in cumulus cells and oocytes. (A) Representative images of the ROS levels detected by flow cytometry after cumulus cells were treated with different doses of AKG, n = 5. (B) Bar graphs showed the quantification of ROS levels. (C) The original graph of Flow cytometry of Annexin/PI double stained of cumulus cells. The graph showed live cells in Q4, early apoptotic cells in Q3, late apoptotic cells in Q2. (D) Bar plots show population of viable (annexin V−/PI−), (E) early apoptotic (annexin V+/PI−), (F) late apoptotic (annexin V+/PI+) and (G) necrotic (annexin V−/PI+) cells. (H) Representative images of DCFHDA staining in control (n = 30), AKG30 (n = 31), and AKG750 (n = 29), Scale bar = 100 µM. (I) Bar graphs showed the quantification of ROS levels. (J) Representative images of Annexin-V staining apoptotic status in control (n = 25), AKG30 (n = 25), and AKG750 (n = 30), Scale bar = 40 µM. (K) Bar graphs showed the quantification of apoptosis levels