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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: The dose-dependent dual effects of alpha-ketoglutarate (AKG) on cumulus oocyte complexes during in vitro maturation

Fig. 4

Effect of AKG supplementation on oocyte mitochondrial distribution and function. (A) Representative images of mitochondria distribution labeled with MitoTracker Red, Scale bar = 40 µM. (B) The proportion of abnormally distributed mitochondria, n = 30. (C) Oocyte ATP levels, n = 3. (D) Representative images stained with JC-1 indicated MMP levels in control (n = 27), AKG30 (n = 27), and AKG750 (n = 25). (E) The statistical data from (D). (F) GO-BP pathway analysis of differential genes between both AKG concentrations vs. control group, color represents -lg(P-value), and circle size represents fold enrichment. (G) Immunoblotting of NDUFB9 in control, 30 µM AKG, and 750 µM AKG treated oocytes. (H) The expression of mitochondrial respiratory chain complex I related genes is expressed by log2(TPM + 1), n = 8

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Cell Communication and Signaling

ISSN: 1478-811X