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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: The dose-dependent dual effects of alpha-ketoglutarate (AKG) on cumulus oocyte complexes during in vitro maturation

Fig. 6

Effects of AKG and GDF9 on cumulus cells expansion. (A) The expression of cumulus expansion related genes HAS2, PTX3, CD44 and HMMR in cumulus cells expressed by log2 (TPM + 1), n = 3. (B) Immunoblotting of HAS2 in control, 30 and 750 µM AKG treated cumulus cells. (C) Representative images of the COCs after 22 h of culture, Scale bar = 200 µM, n = 30. (D) Relative cumulus expansion area n = 30. (E) Cumulus expansion index, n = 30. (F) qPCR results showed the expression of cumulus expansion related genes HAS2, PTX3, CD44 and HMMR in cumulus, n = 3

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Cell Communication and Signaling

ISSN: 1478-811X