Fig. 4

USP11 deficiency attenuates lipogenesis in HCC cells. A. Oil red O staining (Upper) and Nile red staining (Lower) were used to evaluate the formation of lipids in HCC-LM3 cells transfected with control siRNA or USP11 siRNA. Scale bars, 20 μm. B. Oil red O staining (Upper) and Nile red staining (Lower) were employed to assess lipid formation in Huh7 cells transfected with Vector or USP11. Scale bars, 20 μm. C. Triglycerides (Left panel) and cholesterol (Right panel) in HCC-LM3 cells transfected with control siRNA or USP11 siRNA were quantified using tissue-cell assay kits for triglycerides and total cholesterol. D. Triglycerides (Left panel) and cholesterol (Right panel) in Huh7 cells with USP11 overexpression were quantified using tissue-cell assay kits for triglycerides and total cholesterol. E. Western blot detection of USP11, ACLY, FASN, ACACA, and SCD1 protein expression in USP11-knockdown HCC-LM3 cells. F. Western blot detection of USP11, ACLY, FASN, ACACA, and SCD1 protein expression in Huh7 cells with USP11 overexpression. G, H. RT‒qPCR were utilized to measure the mRNA levels of ACLY, FASN, ACACA, and SCD1 in HCC-LM3 and Huh7 cells. *p < 0.05, **p < 0.01, ***p < 0.001, **** p < 0.0001