Fig. 4
From: Phosphorylation of Bok at Ser-8 blocks its ability to suppress IP3R-mediated calcium mobilization
Exogenous Bok regulates exogenous IP3R1-mediated [Ca2+]C responses in HEK-3KO cells. (A) HEK-3KO cells were transfected to express IP3R1HAWT or IP3R1HAΔ1916/17 (2 µg cDNAs) with BokWT or vector (0.125 µg cDNA). Lysates were probed in immunoblots as indicated, with p97 serving as a loading control. (B) [Ca2+]C (F/F0) in transfected HEK-3KO cells exposed to 100 µM CCh, added at t = 0. Parallel analysis of non-transfected HEK-3KO cells shows that [Ca2+]C responses are due to exogenous IP3R1 expression. (C) Maximal F/F0 values (Fmax) (mean ± SEM, n = 3, ns = not significant, p > 0.05). (D) Post-maximal decline in [Ca2+]C graphed as F/Fmax. (E) and (F) Post-maximal area under the curve (AUC) and time to F/Fmax = 0.6 (mean ± SEM, n = 4, *** designates p < 0.0005, ns = not significant, p > 0.05)