Fig. 4
From: NKX6.3 modulation of mitotic dynamics and genomic stability in gastric carcinogenesis
Molecular insights into NKX6.3-associated cellular alterations revealed by sequencing analysis. a GSEA illustrating the molecular upheavals underlying cellular irregularities induced by NKX6.3 depletion. b GO analysis identifies biological processes significantly altered by NKX6.3 depletion. The table displays selected GO terms with their corresponding log2 fold change and adjusted p-value. c Heatmap depicting expression changes of key mitotic spindle-associated genes in HFE-145shCtrl, HFE-145shNKX6.3#1 and HFE-145shNKX6.3#2 cells. Genes like AurkA and TPX2 show significant upregulation in NKX6.3-depleted cells. d GSEA enrichment plots for the HALLMARK_MITOTIC_SPINDLE gene set across experimental data and TCGA_STAD dataset. Both datasets show significant positive enrichment, indicating the conserved role of identified genes in mitotic spindle function. e Gene network analysis revealing central hub genes, such as AurkA and TPX2, crucial to mitotic nuclear division, spindle assembly, and the G2/M phase transition