Fig. 6
From: NKX6.3 modulation of mitotic dynamics and genomic stability in gastric carcinogenesis
Regulatory Impact of NKX6.3 on AurkA and TPX2 Expression and Mitotic Integrity in Xenograft Mouse Models. a and b Relative DNA copy number (a) and mRNA expression (b) levels of AurkA and TPX2 in NKX6.3 depleted cells injected xenograft mouse models compared to HFE-145shCtrl cells. c Western blot and ELISA analysis showing protein expression levels of AurkA and TPX2 in HFE-145shNKX6.3 xenograft mice and HFE-145shCtrl cells. d Histological examination by H&E staining revealing cellular morphology changes and immunohistochemical analysis for NKX6.3, AurkA, TPX2, and Ki-67 markers highlighting regulatory effects. e Representative H&E staining of xenograft tumor sections from NKX6.3-depleted cells injected tumor nodule. f Quantification of aberrant mitotic figures in NKX6.3-depleted cells injected xenograft tumor sections. All data are presented as mean ± SD of three independent experiments. Asterisk indicates **** p < 0.0001