Fig. 7
From: NKX6.3 modulation of mitotic dynamics and genomic stability in gastric carcinogenesis
NKX6.3 expression is inversely correlated with AurkA and TPX2 overexpression and mitotic abnormalities in gastric cancer. a Upregulation of AurkA and TPX2 mRNA expression in human gastric cancers (n = 65). b Correlation between DNA copy number variations and mRNA expression of AurkA and TPX2 genes. Y-axis denotes relative mRNA expression (fold change), X-axis categorizes DNA copy number status (Shallow Deletion, Diploid, Gain/Amplification). c-e Inverse correlation between NKX6.3 protein expression and AurkA/TPX2 protein levels (c), DNA copy number (d), and mRNA expression (e) in gastric cancer tissues. f Immunohistochemical staining showing enhanced protein levels of AurkA and TPX2 in NKX6.3 negative gastric cancer tissues compared to NKX6.3 positive tissues. g Comparative analysis of mitotic integrity via H&E staining revealed a decrease in abnormal mitotic figures and the abnormal mitotic index in NKX6.3-positive gastric cancer tissues. All data are presented as mean ± SD of three independent experiments. Asterisk indicates **** p < 0.0001