Fig. 4

Expression patterns and subcellular localization of amhr2 transcripts. A Expression of tissue specificity and early development of amhr2 mRNA transcript variants. a-d. Tissue distribution of amhr2 mRNA transcript variants. Different letters indicate that the expression levels in different tissues were significantly different. T: testis; O: ovary; B: brain; L: liver; K: kidney; I: intestine; M: muscle; St: stomach; G: gill; Sk: skin; H: heart; S: spleen; E: eye. e–h. Relative expression of amhr2 mRNA transcript variants from 5 to 360 dph. *, P < 0.05. B Localization of amhr2 in the ovary and testis. a, d: The testis and ovary of Spotted knifejaw. b: The respective locations amhr2 labeled with antisense probes in the testis; e: The locations of amhr2 in the ovary; c, f: negative controls labeled with each sense probe in the testes or ovary. Sz: spermatozoa; Ssp: Secondary Spermatocyte; Psp: Primary spermatocyte; Ep: early perinucleolus stage oocytes; Lp: late perinucleolus stage oocytes; Se: Sertoli cells. Scale bar: a-f: 50 µm