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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Dose-dependent role of AMH and AMHR2 signaling in male differentiation and regulation of sex determination in Spotted knifejaw (Oplegnathus punctatus) with X1X1X2X2/X1X2Y chromosome system

Fig. 6

Functional validation of the amhr2 gene including knockdown and overexpression. A RNA interference of amhr2 gene. a: Schematic diagram of amhr2 RNA knockdown. b: Changes in the expression of sex-related genes after amhr2 RNA interference on male testes. dsRNA: dsRNA treatment group, gonad cells treated with the amhr2 antisense probe; NC: negative control group, gonad cells treated with the amhr2 sense probe. The asterisk (*) indicates statistically significant intergroup differences (P < 0.05). B Overexpress of amhr2 gene. a: Recombinant bacteria amhr2 band amplification; b: Western blot verification of Amhr2 protein. c: Changes in the expression of sex-related genes after amhr2 overexpression on male testes. amhr2: amhr2 recombinant plasmid treatment group; control: gonad cells treated with PBS buffer. The asterisk (*) indicates statistically significant intergroup differences (P < 0.05)

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Cell Communication and Signaling

ISSN: 1478-811X