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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Increased melanin induces aberrant keratinocyte − melanocyte − basal − fibroblast cell communication and fibrogenesis by inducing iron overload and ferroptosis resistance in keloids

Fig. 5

Melanin inhibits ferroptosis in KD fibroblasts. (A-C) qPCR and western blot detection of ferroptosis-inhibitory proteins SLC3A2/SLC7A11/GPX4 and pro-ferroptotic proteins in KD fibroblast cells with or without melanin stimulation. (D) The mRNA levels of ACSL4, SLC7A11, GPX4, and SLC3A2 in KD and KN skin tissues by scRNA-seq analysis. (E) Evaluation of the effects of melanin on RSL-3-induced LipidROS in KD fibroblast cells. Scale bar: 200 μm. (F) Cell viability of fibroblast cells treated with RSL-3 and melanin by CCK8 assay. (G-H) Relative 4-HNE and MDA levels after fibroblast cell treated withRSL-3 and melanin. (I-J) Western blot analysis of SLC3A2, SLC7A11, GPX4, and ACSL4 protein levels in KN and KD primary fibroblasts. N = 6. (K-L) Immunofluorescence assay and cell count of GPX4+S100A4+ fibroblast cells in keloid skin tissues. N = 6. Scale bar: 200 μm. The experiments in A-H were repeated three times independently. *P < 0.05; **P < 0.001; ***P < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X