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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Increased melanin induces aberrant keratinocyte − melanocyte − basal − fibroblast cell communication and fibrogenesis by inducing iron overload and ferroptosis resistance in keloids

Fig. 6

Melanin-induced iron overload and ferroptosis-resistance in melanocytes and benefited keratinocyte cells absorbing melanin. (A-C) The expression of iron metabolism-related genes after 100 ug/ml melanin incubation in pHEMs. (D) Total iron, Fe2+, and Fe3+ levels in melanin-treated melanocytes. (E) Cell viability of pHEMs treated with RSL-3 and melanin by CCK8 assay. (F-H) qPCR and western blot detection of ferroptosis-inhibitory proteins SLC3A2/SLC7A11/GPX4 and pro-ferroptotic proteins in pHEMs with or without melanin stimulation. (I) Perl’s staining of iron content in epidermal skin tissues. Scale bar: 50 μm. (J) Evaluation of the effects of melanin on RSL-3-induced LipidROS in pHEMs. Scale bar: 200 μm. (K) Melanin contents in HaCaT cells. (L-N) qPCR and western blot analysis of PAR-2 protein levels in HaCaT cells. The experiments were repeated three times independently. *P < 0.05; **P < 0.001; ***P < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X