Fig. 2

Mechanosensory apparatus in EOC: PIEZO1 activity (A) Illustrative curves of intracellular Ca²⁺ levels as function of time with the first increase after YODA1 (5 µM) treatment highlighted in box (B) Immediate increase (1 min) in intracellular Ca²⁺ levels in cells treated with YODA1 (5 µM) with or without pre-treatment with YODA1 (1 µM) for 24 h. Increase is expressed as percentage of increase/basal levels ratio. * represents the difference between treatment and respective control, # represents differences between cell lines. (C) Total increase (23 min) in intracellular Ca²⁺ levels in cells treated with YODA1 (5 µM) with or without pre-treatment with YODA1 (1 µM) for 24 h. Increase is expressed as percentage of increase/basal levels ratio. * represents the difference between treatment and respective control, # represents differences between cell lines. (D) Quantification of PIEZO1 expression [r.f.u.] at the plasma membrane in cells exposed to SS for 3 h and 24 h and in respective static controls. All experiments were performed in at least 3 independent biological replicates. Statistical significance calculated with t-test and one-way ANOVA, and shown as */# p < 0.05, **/## p < 0.01, ***/### p < 0.001