Fig. 3
From: Glucose- and glutamine-driven de novo nucleotide synthesis facilitates WSSV replication in shrimp
WSSV increased 13C-labeled metabolites in the de novo nucleotide synthesis pathway at the WSSV replication stage (12 hpi). (a) Schematic indicating how metabolites derived from [U-13C]glucose can be traced through the de novo nucleotide synthesis pathway. White circles represent 12Carbon, red circles represent 13Carbon, and yellow asterisks indicate the targets that were traced. At (b) 12 h and (c) 24 h post injected with WSSV or PBS, shrimp were injected with [U-13C]glucose and hemocytes were collected 10–30 minutes later. Metabolomic analysis of pooled hemocyte samples by LC-ESI-Q-TOF-MS was used to calculate the fold change of each 13C metabolite in the WSSV group compared to the corresponding 13C metabolite in the PBS group. Each bar represents the mean ± SD. Asterisks indicate differences between the WSSV group and the corresponding PBS control group (* p < 0.05, ** p < 0.01). Summary of changes in 13C-labeled metabolites in the nucleotide synthesis pathway at (d) 12 hpi and (e) 24 hpi, 10 minutes after [U-13C]glucose treatment. Changes in the WSSV group relative to the corresponding PBS control are color-coded as follows: red (significant increase), green (significant decrease), yellow (no significant change), and white (not detected)