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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Glucose- and glutamine-driven de novo nucleotide synthesis facilitates WSSV replication in shrimp

Fig. 6

Down-regulation of LvTKT, LvPRPS, LvIMPDH, LvCAD, LvDHODH, LvUMPS, and LvCMPK mRNA by specific dsRNA injection and its effect on WSSV IE1, VP28 gene expression and WSSV genome copy number. Gene silencing efficiency at (a) 24 h post WSSV injection. WSSV (b) IE1, (c) VP28 gene expression and (d) genome copy number were evaluated after gene silencing of LvTKT, LvPRPS, LvIMPDH, LvCAD, LvDHODH, LvUMPS, and LvCMPK. Groups treated with PBS only or with non-specific luciferase (Luc) dsRNA were used as control groups. Each bar represents the mean ± SD of mRNA expression. Asterisks indicate differences between the WSSV group and the corresponding control group (* p < 0.05, ** p < 0.01)

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Cell Communication and Signaling

ISSN: 1478-811X