Fig. 4

PC treatment induces mitochondrial dysfunction, ROS and inhibits mtDNA in CC. A Representative confocal microscopy images showing the changes in mitochondrial morphology and its distribution in PC-treated SiHa and HeLa cells. B Representative confocal microscopy images of PC-treated SiHa and HeLa cells stained with Rhodamine-123. Bar graphs illustrate the dose-dependent elevation in MMP in PC-treated SiHa and HeLa. C The bar graph represents the mtDNA content analysis. The bar graph represents the ratio between mtCOX and nuclear β-Actin. D Represents the DCFDA stained control and PC exposed SiHa and HeLa cells. E The bar graph represents the mean fluorescence intensity of the DCFDA in control and PC-exposed SiHa and HeLa cells. F Represents the mitoSOX stained control and PC-exposed SiHa and HeLa cells. G The bar graph represents the mean fluorescence intensity of the mitoSOX in control and PC-exposed SiHa and HeLa cells. *P < 0.05, **P < 0.01, and ***P < 0.001 indicates statistical significance