Fig. 1

Dynamic changes in hepatic macrophage populations of mice with diet-induced MASH. (A) Representative density plots of liver macrophage populations at indicated feeding periods. After gating on CD45⁺, live and singlet cells, macrophages were identified as F4/80⁺ cells (top panel) and further analysed for expression of Tim4 and Clec4F (bottom panel). (B) Quantification of F4/80⁺ cells per milligram liver tissue. (C) Proportion of Clec4F⁺Tim4⁺ Kupffer cells (KC), Clec4F⁻Tim4⁻ monocyte-derived macrophages (MoMF), and Clec4F⁺Tim4⁻ monocyte-derived Kupffer cells (MoKC) as fractions of F4/80⁺ cells. Values are median (line), upper- and lower quartile (box) and extremes (whiskers) of n = 4–8 (STD), n = 8–11 (4 W MASH-D), n = 8–13 (12 W MASH-D), n = 9–11 (20 W MASH-D) mice. Statistics: One-way-ANOVA with Tukey´s post hoc test for multiple comparison. *p < 0.05