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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Dynamic changes in macrophage populations and resulting alterations in Prostaglandin E2 sensitivity in mice with diet-induced MASH

Fig. 2

Modulation of TNF-α mRNA and protein expression by exogenous PGE2. Primary Kupffer cells (KC), peritoneal macrophages (PM) and bone marrow-derived macrophages (BMDM) were stimulated for 24 h with LPS and/or PGE2. (A) Relative mRNA expression of TNF-α was determined by RT-qPCR with Hprt as the reference gene. (B) TNF-α protein levels were quantified by ELISA in cell culture supernatants. Values are median (line), upper- and lower quartile (box) and extremes (whiskers) of (A) n = 33 (KC), n = 26 (PM) or n = 29 (BMDM) or (B) n = 27 (KC), n = 26 (PM) or n = 26 (BMDM) independent experiments. Statistics: Two-way-ANOVA with Tukey´s post hoc test for multiple comparison. *p < 0.05

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Cell Communication and Signaling

ISSN: 1478-811X