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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Dynamic changes in macrophage populations and resulting alterations in Prostaglandin E2 sensitivity in mice with diet-induced MASH

Fig. 3

PGE2 inhibits TNF-α mRNA and protein expression in a dose-dependent manner. Primary Kupffer cells (KC), peritoneal macrophages (PM) and bone marrow-derived macrophages (BMDM) were stimulated for 24 h with LPS and indicated concentrations of PGE2. (A) Relative mRNA expression of TNF-α was determined by RT-qPCR with Hprt as the reference gene. (B) TNF-α protein levels were determined by ELISA in cell culture supernatants. Values are mean ± SEM of n = 5 independent experiments. Statistics: Student´s t-test for unpaired samples. * vs. w/o PGE2 with p < 0.05

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Cell Communication and Signaling

ISSN: 1478-811X