Fig. 2
DME-primed DCs induce Th2 cell development. BALF and single cells were isolated from the lungs of mice, which were treated with nasal instillations containing DME or PBS for 5 days. A-C, bars show the quantities of Th2 cytokines in BALF. D-G, single cells were analyzed using flow cytometry. D, adhesive cells were gated out. E, CD3+CD4+ T cells were gated, from which Th2 cells were gated (F). G, bars show the counts of Th2 cells in F. H-I, lines show Mettl5 mRNA (H) or Mettl5 protein in DCs after exposure of them to DME in culture for 2 days. J, preparing Mettl5-expressing DCs (by exposing to DME or PBS in culture for 2 days). K-O, the DME (or PBS)-pulsed DCs were cultured with naïve CD4+ T cells for 3 days. Cells were analyzed using flow cytometry. K, CD4+ T cells were gated, from which the induced Th2 cells were gated (L-Q, the gated flow cytometry plots show Th2 cells; the bars show the counts of Th2 cells). The data of bars are presented as mean ± SD. Each bubble in bars presents one sample (assessed in triplicate). Statistics: ANOVA + Tukey HSD test (A-C, G) and Student’s t-test (M, O, Q). *p < 0.05; **p < 0.01; ***p < 0.001. KO: Mettl5f/fItgax-Cre mice. cKO: Mettl5f/f mice. Each group consists of 6 mice. DME: Dust mite extracts