Fig. 6

CYP2E1 expression affects the morphology and function of mitochondria under DXR stimulation. (A) Schematic diagram illustrating the experimental strategy to analyze the role of CYP2E1 in mitochondrial morphology and OPA1 processing in myocardium-specific Cyp2e1-KO and Cyp2e1-OV rats under pathological myocardial injury. (B) TEM images of myocardium from control-DXR (CON-DXR), Cyp2e1-OV-DXR (OV-DXR), and Cyp2e1-KO-DXR (KO-DXR) groups. Blue and purple arrowheads indicate smaller and elongated mitochondria, respectively. (C-D) Histogram and violin plot presenting the frequency distribution and median surface area of mitochondria across the three groups (49–67 mitochondria were analyzed per group). CSA, cell cross-sectional area. (E-G) Levels of CYP2E1 proteins, phosphorylated Drp1 (phosphor-Drp1) at Ser616 and Ser637), and Drp1 translocation (mitochondria and cytoplasm) were quantified via immunoblotting and standardized to GAPDH or VDAC1. Data shown are mean ± SEM; n = 4–6 per groups. (H-I) Activity of mitochondrial Complex I and mitochondrial Complex II. Data shown are mean ± SEM; n = 4–7 per groups. (J-K) Levels of NDUFS1, SDHA, SDHB and CYTB were quantified via immunoblotting, and standardized to GAPDH. Data shown are mean ± SEM; n = 4–6 per groups. Multiple comparisons between groups were analyzed using ANOVA with Tukey correction. *P < 0.05, **P < 0.01, ***P < 0.001